Effect of MAL-silencing on TCRζ, ZAP70, LAT, and SLP76 phosphorylation levels. (A–E) Primary CD4 T cells were transfected with a siRNA control (siCtr; J–L), or siRNA MAL (siSyt7; M–O) and allowed to form conjugates with superantigen-loaded Raji cells for 30 min. Cells were stained pLck (A), pTCRζ (B), pZAP70 (C), pLAT (D), or pSLP76 (E), and cellular levels were determined by intracellular fluorescence cytometry. Representative of two experiments.