Lck, TCRζ, and LAT traffic through distinct exocytic compartments. Jurkat cells were transfected with GFP-tagged Rab3d (A, I, and Q), Rab4b (B, J, and R), Rab8b (C, K, and S), Rab11b (D, L, and T), Rab27a (E, M, and U), Rab37 (F, N, and V), Ti-VAMP (G, O, and W), and MAL (H, P, and X). Cells were then stained for Lck (A–H), LAT (I–P), and TCRζ (R–X). 3D confocal images were post-treated by deconvolution. A 1-µm-thick medial stack is shown. Right panels show a zoomed image of the vesicular compartment (frame). Plots in the far right column depict the population analysis of the co-localization volume between Lck, LAT, and TCRζ and each one of the traffic regulators analyzed for at least 20 cells per group. ***, P ≤ 0.0001; **, P ≤ 0.01; *, P ≤ 0.05; Mann-Whitney test. Images representative of three experiments. Bar, 5 µm.