PDGF-B is up-regulated in the tumor endothelium upon Wnt1 expression. (A) Representative ISH for Pdgfb of subcutaneous Wnt1^+/−D tumors. Asterisks indicate vessels, and arrowheads point to Pdgfb⁺ ECs. Insets show representative vessels in higher magnification. Bars: (full images) 50 µm; (insets) 18 µm. (B, left) Representative picture of isolated subcutaneous tumor vessels and dot blot for von Willebrand factor (vWF). (right) Representative Western blot for PDGF-B with pooled subcutaneous tumors vessels (n = 2 tumors/group). Densitometric analysis for PDGF-B of five independent Western blot experiments. Error bars indicate SEM.