Vascular effects observed in Wnt1-expressing gliomas are EC autonomous. (A, left) IF staining for β-galactosidase (β-gal), Podxl, and TOPRO-3 on GL261 glioma sections from Pdgfb-iCreERT2 × ROSA26^STOPfloxLacZ mice treated with tamoxifen. (right) Quantification of β-gal⁺ vessels. (B, left) IF staining for Podxl and TOPRO-3 on GL261 glioma sections from Pdgfb-iCreERT2 (control) and Pdgfb-iCreERT2 × βCat^Ex3flox (GOF) mice. (right) Quantification of Podxl⁺ areas within tumors (n = 4 tumors/group, 10 pictures/tumor; *, P < 0.05). (C, left) ColIV and Podxl staining of tumor sections from control and GOF mice. Arrowheads point to nonendothelial-associated ColIV⁺ structures. (right) ColIV/Podxl ratio (n = 4 tumors/group, 8 pictures/tumor; *, P < 0.05). (D, top left) IF staining for CD31/PECAM-1 and desmin on tumor sections from control and GOF mice (n = 4 tumors/group, 10 pictures/tumor). (right) Quantification of PC coverage (***, P < 0.001). (bottom left) Representative vessel in higher magnification (white boxes). Bars: (A [left] and D [top]) 160 µm; (A, right) 40 µm; (B) 400 µm; (C and D [bottom]) 20 µm. Error bars indicate SEM.