Neutrophil mobilization patterns of WHIM-LysM-GFP and WT-mT/mG mice. (a) The experimental procedure involved selection of Ly6G⁺ cells from BM cells isolated from donor WHIM-LysM-GFP and mT/mG mice. Cells were subsequently i.v. transferred in a 1:1 ratio into WT recipient mice pretreated for 1 h s.c. with PBS or plerixafor. 2 h after transfer of fluorescent-tagged cells, blood and perfused femur were collected. (b, Left) The normalized number of circulating fluorescent-tagged neutrophils 2 h after transfer. (b, Right) The normalized number of fluorescent-tagged neutrophils detected in the recipient BM (n = 4–5 per group). A representative set of data were shown from two independent experiments. All data are shown as mean ± SEM. Student’s two-tailed unpaired t test was performed. ***, P < 0.001.