Neutrophil egress in LysM-GFP and WHIM-LysM-GFP mice. (a) Mean neutrophil migratory velocities at 25 min after G-CSF injection in skull BM of LysM-GFP and WHIM-LysM-GFP mice (5-min tracking period, n = 3 per group). Each symbol corresponds to an individual neutrophil tracked and the bars represent mean migratory velocity of each group. A Student’s two-tailed unpaired t test was performed. n.s. = not significant. (b) Displacement of migrating GFP⁺ neutrophils (relative to their initial positions) in LysM-GFP and WHIM-LysM-GFP mice during basal (left) and 25 min after G-CSF injection (right; 5-min tracking period, n = 3 per group). All data are shown as mean ± SEM. (c) Relative GFP fluorescence intensity in the skull BM parenchymal region in LysM-GFP and WHIM-LysM-GFP mice treated with G-CSF (left) or plerixafor (right) over time (representative of three independent experiments).