Figure 6.
Figure 6. CSB-deficient cells show accumulation of damaged mitochondria. (A) Representative electron microscopy images of CS1AN cells lines after treatment with vehicle or 5 µM rotenone. Red dots denote swollen/damaged mitochondria (n = 12). (B) Quantification of damaged mitochondria in vehicle and rotenone-treated CS1AN cells expressing WT CSB or an empty vector (n = 12; data are represented as mean ± SEM). (C) Mitochondrial diameter in CS1AN cells expressing WT CSB or an empty vector after vehicle or rotenone treatment. Each point represents one mitochondria and cross bar represents mean ± SD. (D) Arrows marking hyperdense membranous material in CS1AN cells expressing an empty vector. (E) Arrows marking mitochondrial onion formation in CS1AN cells expressing an empty vector. (F) ROS formation measured by dihydroethidium in CS1AN cells expressing WT CSB or an empty vector (n = 10; data are represented as mean ± SD).

CSB-deficient cells show accumulation of damaged mitochondria. (A) Representative electron microscopy images of CS1AN cells lines after treatment with vehicle or 5 µM rotenone. Red dots denote swollen/damaged mitochondria (n = 12). (B) Quantification of damaged mitochondria in vehicle and rotenone-treated CS1AN cells expressing WT CSB or an empty vector (n = 12; data are represented as mean ± SEM). (C) Mitochondrial diameter in CS1AN cells expressing WT CSB or an empty vector after vehicle or rotenone treatment. Each point represents one mitochondria and cross bar represents mean ± SD. (D) Arrows marking hyperdense membranous material in CS1AN cells expressing an empty vector. (E) Arrows marking mitochondrial onion formation in CS1AN cells expressing an empty vector. (F) ROS formation measured by dihydroethidium in CS1AN cells expressing WT CSB or an empty vector (n = 10; data are represented as mean ± SD).

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