Figure 9.
![Figure 9. The majority of self-reactive clones have a low TCR affinity for pMHC. (A). HA, MBP, or GAD65 reactive T cells were stained with control CLIP-MHC (MHCII-negative control tetramer) or the appropriate pMHC (MHCII-antigen tetramer) tetramers. (B) 2D affinities measured by the adhesion frequency micropipette assay for clones Ob.1A12, Ob.2F3, Hy.2E11, and HA:D7 (evaluated using the contact area [Ac]: AcKa, µm4). The 2D off-rate (koff, s−1) and the 2D on-rate (Ackon, µm4s−1) were also determined. Data are representative of at least two independent experiments.](https://cdn.rupress.org/rup/content_public/journal/jem/209/2/10.1084_jem.20111485/5/jem_20111485_fig9.jpeg?Expires=1771584393&Signature=uXLbAh~V2gfFnx3sQw6AdWd2rIXpQyOoP1KtouIlizuo8klGMXI1AintIvOKpCFHf9Com2ycHNUt08HESUfeCn-DHaTRW9FADsX4QlXC8mbtlNMP59McZxyJjMt94Mh8dBJ96ttUpglwfJgZ0fw10hcpV7ryT3kjyZ-hs82z5Wk9vlZ8U0B5jmzk-gmvlRCe06mcSTRrLEtNnZQivSGT~Y~tBX8MZ4uf0eEx1RFLFc55OFmYYHp2OEd8J6EMOZwWZ5rBmUY7FVpFWu2ApDgz4FL8W0EzMcVHfly1mLjj9LjWuaCVHYvAmh5xTU~BYcuzcezQyCGHzy3nAumtL5E12A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The majority of self-reactive clones have a low TCR affinity for pMHC. (A). HA, MBP, or GAD65 reactive T cells were stained with control CLIP-MHC (MHCII-negative control tetramer) or the appropriate pMHC (MHCII-antigen tetramer) tetramers. (B) 2D affinities measured by the adhesion frequency micropipette assay for clones Ob.1A12, Ob.2F3, Hy.2E11, and HA:D7 (evaluated using the contact area [Ac]: AcKa, µm4). The 2D off-rate (koff, s−1) and the 2D on-rate (Ackon, µm4s−1) were also determined. Data are representative of at least two independent experiments.
