Figure 4.
Figure 4. Impaired recruitment of TCR-CD3 complexes to synapses formed by self-reactive T cells and APC. (A) T cells were incubated for 30 min with peptide-pulsed EBV-transformed B cells and then fixed and stained for CD3 and LFA-1. Images on the right show an en face view of the contact area. Images for cells from HA:D7, BA-8, Ob.1A12, and Agad303 clones are shown to illustrate the different categories used for classification in B. Bars, 5 µm. (B) Analysis of TCR-CD3 accumulation at the IS in self-reactive and HA-specific T cell clones. For each clone, 100–200 T cell–B cell conjugates were classified as cSMACs, TCR-CD3 clusters (not forming a mature cSMAC), or no TCR-CD3 accumulation. Bar graphs are shown with ± SEM for two to three independent experiments.

Impaired recruitment of TCR-CD3 complexes to synapses formed by self-reactive T cells and APC. (A) T cells were incubated for 30 min with peptide-pulsed EBV-transformed B cells and then fixed and stained for CD3 and LFA-1. Images on the right show an en face view of the contact area. Images for cells from HA:D7, BA-8, Ob.1A12, and Agad303 clones are shown to illustrate the different categories used for classification in B. Bars, 5 µm. (B) Analysis of TCR-CD3 accumulation at the IS in self-reactive and HA-specific T cell clones. For each clone, 100–200 T cell–B cell conjugates were classified as cSMACs, TCR-CD3 clusters (not forming a mature cSMAC), or no TCR-CD3 accumulation. Bar graphs are shown with ± SEM for two to three independent experiments.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal