EAE lesions are characterized by enhanced astrocyte TrkB. (A and B) Immunohistochemistry for TrkB (brown staining, DAB; counterstaining, hematoxylin) in naive (A) and EAE (B) mouse spinal cord. (C–F) Double immunofluorescence for GFAP and the ECD of TrkB (TrkB-ECD in C and D) or the isoform TrkB-T1 (E and F) in naive (C and E) and EAE (D and F) mice. DAPI was used to stain for nuclei. Representative stainings of two naive mice and four EAE mice from two independent EAE experiments are shown. Inset at higher magnification (100× objective, one z plane) shows colocalization. Bars, 50 µm.