Antigen presentation by m/chDCs. (A) Flow cytometry histograms show Y-Ae antibody (line) or isotype (shaded area) staining of gated spleen DCs, brain m/chDCs, and microglia from B6 or BALB/c × B6 (CB1) mice. (B) CFSE-labeled BALB/c T cells were incubated with spleen DCs, brain m/chDCs, or microglia (MG) isolated from B6 mice treated with Flt3L and were analyzed with flow cytometry 4 d later. Graph shows percentage of proliferated T cells among total CD3⁺ T cells, indicated by dilution of CFSE. One of three representative experiments is shown. (C) CFSE-labeled 2D2 MOG-specific CD4⁺ transgenic T cells were incubated with spleen DCs, brain m/chDCs, or microglia isolated from B6 mice treated with Flt3L with the indicated doses of MOG peptide and were analyzed by flow cytometry 4 d later. Graph shows percentage of proliferated (CFSE^lo) T cells among total CD3⁺ T cells. One of three representative experiments is shown. (B and C) Error bars show the SD between triplicate wells.