CD8+ T cells rapidly scan infected non-DCs. (A) MPM time series of T cells interacting with infected DCs, uninfected DCs, or infected non-DCs. CD11c-eYFP mice were given 107 Cell Tracker Blue-labeled OT-I cells (blue) and were infected s.c. with VV-NP-S-mCherry 12 h. later. Six hpi, inguinal LNs were imaged over sequential 20–30 min periods using MPM. The tracks of individual T cells during a 20-min imaging period are plotted (right). (B) T cell speeds, (C) track straightness (T cell displacement/track length), and (D) T cell arrest calculated for each type of APC interaction over 30 min. Open circles, infected DCs; closed triangles, uninfected DCs; closed circles, macrophages. Mean and SEM is shown. Statistics were performed using an unpaired Student’s t test. Shown is one experiment of two analyzed with 10–20 nodes per group.
