CD8⁺ T cells interact with LN DCs and macrophages after viral infection. (A) LNs from CD11c-eYFP mouse (green DCs) excised 10 hpi with VV-NP-S-mCherry (red) were examined as whole mounts by confocal microscopy. OT-I cells were labeled with Cell Tracker Blue before transfer. (B) MPM images of the inguinal LN, as in A. (C) MPM images from the top 30 µm of the inguinal node of CD11c-eYFP mice given fluorescent-dextran (red) to label macrophages and OT-I cells (blue). Uninfected nodes (left) 10 hpi with nonfluorescent backbone virus (VV-β-gal; control, middle left) or VV-Ova (middle right). (right) A schematic of T cell localization in relation to macrophages after VV infection. DCs (green), MRR (red), OT-I cells (blue; right). Scale bars are shown in micrometers. We made similar observations in two additional experiments.