Figure 5.
Figure 5. Platelet production is abnormal in the absence of Bcl-xL. (a) Representative transmission electron microscope images of BM sections showing aberrant MGK morphology and abortive platelet shedding in Bcl-xPf4Δ/Pf4Δ mice. Arrows indicate normal platelets in wild-type and large megakaryocyte-derived fragments in Bcl-xPf4Δ/Pf4Δ animals. Red blood cell (R). Bars: 5 or 10 µm, as indicated. See Fig. S3 for higher magnification images. (b) Mice reconstituted with a 50:50 mixture of Bcl-xPf4Δ/Pf4Δ and wild-type GFP-expressing BMCs. (left) Platelet counts in peripheral blood 7 wk after transplant, mean ± SEM. (middle) Percent CD41+ve GFP+ve platelets; mean ± SD. n = 6. (right) Flow cytometric forward and side scatter plots of CD41+ve platelets in PRP. In these mice, GFP+ve platelets were produced by wild-type MGKs, GFP−ve platelets by Bcl-xPf4Δ/Pf4Δ MGKs. Plots are representative of n = 6 recipient mice per transplant group.

Platelet production is abnormal in the absence of Bcl-xL. (a) Representative transmission electron microscope images of BM sections showing aberrant MGK morphology and abortive platelet shedding in Bcl-xPf4Δ/Pf4Δ mice. Arrows indicate normal platelets in wild-type and large megakaryocyte-derived fragments in Bcl-xPf4Δ/Pf4Δ animals. Red blood cell (R). Bars: 5 or 10 µm, as indicated. See Fig. S3 for higher magnification images. (b) Mice reconstituted with a 50:50 mixture of Bcl-xPf4Δ/Pf4Δ and wild-type GFP-expressing BMCs. (left) Platelet counts in peripheral blood 7 wk after transplant, mean ± SEM. (middle) Percent CD41+ve GFP+ve platelets; mean ± SD. n = 6. (right) Flow cytometric forward and side scatter plots of CD41+ve platelets in PRP. In these mice, GFP+ve platelets were produced by wild-type MGKs, GFP−ve platelets by Bcl-xPf4Δ/Pf4Δ MGKs. Plots are representative of n = 6 recipient mice per transplant group.

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