Level of c-MYC expression affects INK4A/ARF locus genes and genes related to MK maturation during megakaryopoiesis from pluripotent stem cells. qRT-PCR analysis of total c-MYC (A, endogenous plus exogenous), p14ARF (B), p16INK4A (C), GATA1 (D), β1-tubulin (E), and NF-E2 p45 (F) expression in hESCs, with and without overexpression (O/E) of exogenous c-MYC, on days 22 and 26 (7 and 11 d after transduction) in three-factor hiPSCs (TkDN4-M) or in four-factor hiPSCs (TkDA3-4 and TkDA3-5) on days 0, 15, 22, and 26. All levels were normalized to the level of GAPDH expression (n = 4 of two independent samples). The levels of c-MYC (A), p14ARF (B), and p16INK4A (C) expression in an undifferentiated TkDA3-4 iPSC clone (day 0) or expression of the other genes (D–F) in TkDA3-4–derived mature MKs (day 26) was assigned a value of 1.0 (n = 4, means ± SEM).