Effects of reprogramming factors on megakaryopoiesis. (A–C) Each reprogramming factor was transduced, together with EGFP or KO markers, on day 15 of MK-lineage culture. Numbers of total and marker genes EGFP or KO-expressing cells in floating cells on day 17 (A) and CD42b⁺ MKs on days 22 (B) and 26 (C) were measured (n = 3, means ± SEM). (D) Representative flow cytometry dot plots of hESC-derived hematopoietic cells transduced with vehicle (EGFP), OCT3/4-KO, SOX2-EGFP, KLF4-EGFP, or c-MYC-EGFP on day 22. (E and F) On day 22, May-Giemsa staining (E) or ploidy analysis (F) of the cells transduced with vehicle or c-MYC was examined.