Dynamics of MTOC polarization in DCs. (A) Time-lapse video microscopy sequence showing DC-MTOC movements during the interaction of DCs derived from centrin-GFP knock-in mice (the green spots correspond to DCs MTOCs) with OT-I (labeled with SNARF; red). TLR-stimulated DCs were loaded with OVA class I peptide (10 nM) and mixed with OT-I cells. Movies were recorded during the first 50 min of interaction. (B) Stability of MTOC polarization. Bars show the percentage of DC–T cell conjugates (measured on contacts lasting >20 min) in which the DC-MTOC remained oriented during the entire movie length (stable) or moved back to a distal position (transient; 60 cells in four independent experiments were scored).