Figure 5.
Figure 5. Time-lapse quantification of FA dynamics after FLNa inhibition. (A) Control (PSR) and FLNa-silenced (PSR-FLNa) BT-20 cells transfected with EYFP-paxillin were plated on chamber slide, stimulated with 10 ng/ml EGF, and then analyzed by time-lapse spinning disk confocal microscopy. Representative frames are shown. (B) Enlargements of the boxed regions of A showing white arrows that indicate positions of paxillin-containing FAs. (C) Quantification of EYFP-paxillin persistence in FAs. Duration measurements were quantified by counting the amount of time lapsed between the first and last frames in which an individual adhesion was followed. (D) Rate constants of EYFP-paxillin disassembly at individual adhesions were calculated as described in Materials and methods. (C and D) Quantifications show the mean ± SD from three independent experiments (*, P < 0.01). Bars: (A) 15 µm; (B) 5 µm.

Time-lapse quantification of FA dynamics after FLNa inhibition. (A) Control (PSR) and FLNa-silenced (PSR-FLNa) BT-20 cells transfected with EYFP-paxillin were plated on chamber slide, stimulated with 10 ng/ml EGF, and then analyzed by time-lapse spinning disk confocal microscopy. Representative frames are shown. (B) Enlargements of the boxed regions of A showing white arrows that indicate positions of paxillin-containing FAs. (C) Quantification of EYFP-paxillin persistence in FAs. Duration measurements were quantified by counting the amount of time lapsed between the first and last frames in which an individual adhesion was followed. (D) Rate constants of EYFP-paxillin disassembly at individual adhesions were calculated as described in Materials and methods. (C and D) Quantifications show the mean ± SD from three independent experiments (*, P < 0.01). Bars: (A) 15 µm; (B) 5 µm.

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