MOG-specific antibodies in double-transgenic TCR¹⁶⁴⁰ × IgH^MOG and single TCR transgenic mice. (A) MOG binding allotype a autoantibodies were detected by ELISA in sera of indicated groups (each five to six mice). (B) Spontaneous anti-MOG IgG1 allotype b antibodies in TCR¹⁶⁴⁰ and TCR¹⁶⁴⁰ × IgH^MOG but not in NTL (n = 5 for each group). Sera at the indicated dilutions were incubated with plates precoated with rMOG. Bound anti-MOG Ig was detected by allotype-specific antibodies as indicated. Mean absorbance at OD 405 nm is shown. Error bars indicate SEM. Data are representative of two to three independent experiments.