Figure 7.
Figure 7. SMCs express LTβR and TNFRSF1A and induce CXCL13 mRNA. (a) Microarray analyses of LCM-derived media (shaded columns) versus adventitia (open columns) from 78-wk-old wild-type mice were examined for signal strengths of Acta2, Mbp, and Ltbr. Means of three independent LCM preparations of individual aortae ± SEM. *, P < 0.05; **, P < 0.001; two-sided, unpaired Student's t test. (b) qRT-PCR analysis of the expression of Ltbr, Tnfrsf14, Tnfrsf1a, and Tnfrsf1b expression in freshly isolated SMCs. Means of three independent SMC cultures ± SEM. (c) Same analyses as in b in cultured SMCs. (d) Cultured SMCs were stimulated with 10 µg/ml α-LTβR, 1 ng/ml TNF, or both for 24 h, and Cxcl13 transcripts were determined by qRT-PCR. Data represent means of five independent experiments ± SEM. P < 0.05, one-sided paired Student's t test.

SMCs express LTβR and TNFRSF1A and induce CXCL13 mRNA. (a) Microarray analyses of LCM-derived media (shaded columns) versus adventitia (open columns) from 78-wk-old wild-type mice were examined for signal strengths of Acta2, Mbp, and Ltbr. Means of three independent LCM preparations of individual aortae ± SEM. *, P < 0.05; **, P < 0.001; two-sided, unpaired Student's t test. (b) qRT-PCR analysis of the expression of Ltbr, Tnfrsf14, Tnfrsf1a, and Tnfrsf1b expression in freshly isolated SMCs. Means of three independent SMC cultures ± SEM. (c) Same analyses as in b in cultured SMCs. (d) Cultured SMCs were stimulated with 10 µg/ml α-LTβR, 1 ng/ml TNF, or both for 24 h, and Cxcl13 transcripts were determined by qRT-PCR. Data represent means of five independent experiments ± SEM. P < 0.05, one-sided paired Student's t test.

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