TLR ligand–exposed B cells differentiated into CD138 plasma and IgG1-positive B cell. LPS-activated or nonactivated B cells were prepared from GFP mice and transferred into mice immunized 1 wk previously with HEL in CFA. 21 d after immunization, mice were boosted with 100 µg HEL in PBS. 4 wk after boosting, spleen, iLN (immunized and nonimmunized side), and femurs (bone marrow) were removed, gently dissociated into single cell suspensions, and subjected to flow cytometry to analyze GFP, B220, IgG1, or CD138 as indicated. The data shown is mean ± SD from four recipient mice that received non–LPS-activated B cells and four recipient mice that received LPS-activated B cells. The horizontal bars indicate mean values.