Figure 7.
Figure 7. LPS-activated transferred B cells accumulate in the recipient spleens. (A and B) Transferred LPS-activated B cells are found in the B cell follicle and the marginal zone of the spleen. Three groups of five mice (6 wk old, C57BL/6 CD45.2) were injected subcutaneously on day 0 with 100 µg HEL in CFA. 15 million cells of LPS-activated or nonactivated B cells from C57BL/6 CD45.1 mice were injected intravenously to recipient mice on day 7. Mice were boosted on day 28 with 100 µg HEL antigen. On day 38, the mice were sacrificed and the spleens analyzed by immunohistochemistry to detect CD35 and CD45.1 (10× and 20×; A) and to detect MAdCAM-1 versus CD45.1 (20×; B). Bars, 100 µm. The experiment was performed with five mice with similar results.

LPS-activated transferred B cells accumulate in the recipient spleens. (A and B) Transferred LPS-activated B cells are found in the B cell follicle and the marginal zone of the spleen. Three groups of five mice (6 wk old, C57BL/6 CD45.2) were injected subcutaneously on day 0 with 100 µg HEL in CFA. 15 million cells of LPS-activated or nonactivated B cells from C57BL/6 CD45.1 mice were injected intravenously to recipient mice on day 7. Mice were boosted on day 28 with 100 µg HEL antigen. On day 38, the mice were sacrificed and the spleens analyzed by immunohistochemistry to detect CD35 and CD45.1 (10× and 20×; A) and to detect MAdCAM-1 versus CD45.1 (20×; B). Bars, 100 µm. The experiment was performed with five mice with similar results.

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