TLR4 ligand–exposed B cells proliferate in vivo and some adopt a germinal center phenotype. (A) Recovered cell numbers and CFSE profiles of nonstimulated and LPS-stimulated B cells. 10 × 106 cells were labeled with CFSE and transferred intravenously to different recipient mice. At the indicated days, the total number of viable CFSE-labeled cells recovered from the spleen, iLN, and pLN were determined (left). The CFSE profiles of the recovered splenic cells are shown, along with the percentage of cells that divided (right). Data from five recipient mice that received nonactivated B cells and five that received LPS-activated B cells. (B–D) Recovery and phenotype of cells recovered from spleen, bone marrow, and iLN after transfer of LPS-stimulated CD45.1 B cells into CD45.2 mice. Number and phenotype of the recovered CD45.1 cells (left) and CFSE profiles of the CD45.1 cells recovered at day 3 after transfer (right) are shown. Data are from the analysis of five recipient mice that received LPS-activated B cells.
