Comparison of TLR4 ligand–exposed or nonexposed B cells in the iLN by intravital TP-LSM. (A) Imaging. Labeled LPS-activated and control B cells were transferred to recipient mice and the iLN was imaged various days after transfer. A maximum intensity projection (MIP) of a 33-µm image stack shows LPS-activated B cells (green) and nontreated B cells (red). Bars, 50 µm. (B) Zoomed images. An MIP 4× zoom is shown of days 1–3. The day-4 data were obtained using CFSE-labeled and transferred LPS-activated B cells. Arrows point to LN follicle edge. Bar, 12.5 µm. (C) Motility parameters. Each data point represents a single cell and green and red bars indicate mean values (*, P < 0.05; **, P < 0.01). (D) Tracks. Superimposed 20-min tracks of randomly selected cells of indicated types in the x, y plane setting the starting coordinates to the origin. Data are from the analysis of four recipient mice at the indicated times. The experiment was done three times with similar results.