Effect of Ir-CPI on venous thrombosis in rats and mice. (A) Ir-CPI at the indicated doses was administered i.v. 5 min before induction of thrombosis by 10% FeCl3 and complete stasis. The control group received PBS (□) instead of Ir-CPI (▪). Each point represents the mean ± SEM for five or six animals. (B and C) Ir-CPI at the indicated doses was administered i.v. before IVC ligature. The control group received PBS instead of Ir-CPI. The thrombosed IVC fragments were harvested and weighed 24 h later. Results were expressed by dividing the thrombus weight with mouse weight (mg/g; B) or thrombus length (mg/mm; C). (D and E) Time-course experiments of clot formation. Results were expressed by dividing the thrombus weight with mouse weight (mg/g; D) or thrombus length (mg/mm; E). The experiment shown in A is representative of two independent experiments. The data shown in B–E are presented as the mean ± SEM of at least four independent experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (relative to vehicle); #, P ≤ 0.05 (between 1 and 10 mg/kg Ir-CPI dose).
