Cremaster muscle whole mount. Giemsa-stained whole-mounts of TNF-α–treated cremaster muscles of WT, ST3Gal-IV^−/−, and CXCR2^bm−/− mice were analyzed for the number of intravascular (A) and perivascular (B) leukocytes (mean ± SEM per mm² surface area). In addition, two typical micrographs are presented illustrating intra- and perivascular leukocyte distribution in TNF-α–treated cremaster muscle whole mounts of WT control mice (C) and ST3Gal-IV^−/− mice pretreated with E-selectin blocking mAb 9A9 (D). Data in A and B were obtained from at least three independent experiments per group. *, P < 0.05 versus WT mice. Bars, 50 μm.