Differential contributions to LPS-induced liver injury by neutrophil adhesion within sinusoids and postsinusoidal venules. The number of rolling (A) and adherent (B) neutrophils per 100 μm in postsinusoidal venules, the number of adherent neutrophils in sinusoidal capillaries (C), and the percentage of perfused sinusoids (E) were determined by intravital microscopy. (D) Leder-stained histological sections of livers were examined by light microscopy, and positively stained neutrophils were counted per hpf (×400) of view. (F) Serum levels of ALT were measured from whole blood. LPS-treated WT mice, LPS-treated WT mice that received E- and P-selectin–blocking antibodies, and WT or CD44−/− mice that received antibodies to block β2 and α4 integrins were compared with untreated WT mice. Data are presented as arithmetic mean ± SEM of at least five animals per group. *, P < 0.05 relative to untreated controls; #, P < 0.05 relative to LPS-treated WT animals.
