Elimination of DAP12 and FcRγ abolishes E-selectin-mediated downstream signaling in neutrophils. (A and B) Bone marrow–derived neutrophils from WT mice were plated on uncoated (unstimulated) or E-selectin–coated plates for the indicated times, after which lysates were prepared and immunoblotted with antibody to phosphorylated p38 MAPK (phospho-p38) or total p38 (n = 2). Data in B normalized to phospho-p38 in unstimulated WT neutrophils (n = 2). (C) Immunoblot analysis of total cell lysates generated from bone marrow–derived neutrophils of WT mice, Fcrg^−/− mice, Tyrobp^−/− mice, and Tyrobp^−/− Fcrg^−/− mice with antibody to phosphorylated p38 MAPK (phospho-p38) or total p38 (total p38). This was confirmed in four additional experiments.