Gα_i-independent neutrophil recruitment drastically reduced in Tyrobp^−/− Fcrg^−/− mice. Neutrophil influx into the peritoneal cavity 8 h after 1 ml injection of 4% thioglycollate into WT mice (n = 4), Fcrg^−/− mice (n = 3), Tyrobp^−/− mice (n = 7), and Tyrobp^−/− Fcrg^−/− mice (n = 7). The same groups were also analyzed after pretreatment with 4 μg PTx i.v. (+PTx; WT mice + PTx [n = 5], Fcrg^−/− mice + PTx [n = 4], Tyrobp^−/− mice + PTx [n = 7], and Tyrobp^−/− Fcrg^−/− mice + PTx [n = 5]). Number of mice indicated above each bar. Total numbers of neutrophils in the peritoneal lavage fluid determined by flow cytometry and hemocytometer count. ^#, P < 0.05 from all groups by analysis of variance and Newman-Keuls post-hoc test. *, P < 0.05 compared with WT by Student's t test.