The ITAM domain-containing adapters DAP12 and FcRγ are involved in E-selectin–mediated slow rolling in vitro and in vivo. (A) Carotid cannulas were placed in Tyrobp^−/− mice (upsidedown triangle, n = 3), Fcrg^−/− mice (square, n = 3), Tyrobp^−/− Fcrg^−/− mice (triangle, n = 3), and WT mice (filled circle, n = 5) and connected to autoperfused flow chambers. The wall shear stress in all flow chamber experiments was 5–6 dynes/cm². Average rolling velocity of neutrophils on E-selectin (left) and E-selectin and ICAM-1 (right) presented as mean ± SEM. (B) Mixed chimeric mice were generated by injecting bone marrow cells from LysM-GFP⁺ WT mice and Tyrob^−/− Fcrg^−/− mice, mixed at a 1:1 ratio, into lethally irradiated WT mice. Cumulative histogram of rolling velocities of 150 GFP⁺ (WT, filled circle) and 150 GFP⁻ (Tyrob^−/− Fcrg^−/−, open triangle) leukocytes in inflamed cremaster muscle venules of mixed chimeric mice (n = 4) treated with PTx and a monoclonal blocking P-selectin antibody (RB40.34; Video 1). (inset) Mean ± SEM. ^#, P < 0.05. Video 1 is available.