Fgr is required for E-selectin–mediated slow rolling. (A) Carotid cannulas were placed in WT mice (n = 3), hck^−/− mice (n = 3), lyn^−/− mice (n = 3), fgr^−/− mice (n = 3), and hck^−/− lyn^−/− fgr^−/− mice (n = 3) and connected to autoperfused flow chambers. The wall shear stress in all flow chamber experiments was 5–6 dynes/cm². Mean rolling velocity of neutrophils on E-selectin (left) and E-selectin/ICAM-1 (right) presented as mean ± SEM (B) Mixed chimeric mice were generated by injecting bone marrow cells from LysM-GFP⁺ WT mice and fgr^−/− mice into lethally irradiated WT mice. Cumulative histogram of rolling velocity of 121 GFP⁺ (WT, filled circle) and 121 GFP⁻ (fgr^−/−, open diamond) leukocytes in inflamed cremaster muscle venules of mixed chimeric mice (n = 4) treated with PTx and a monoclonal blocking P-selectin antibody. (inset) Mean ± SEM ^#P < 0.05.