Src-family kinases are involved in slow rolling after E-selectin engagement. (A) Rolling velocities of neutrophils from WT mice treated with a specific Src-family kinase inhibitor (PP2) or inactive control (PP3) in E-selectin and E-selectin/ICAM-1–coated autoperfused flow chamber experiments (n = 3). The wall shear stress in all flow chamber experiments was 5–6 dynes/cm². (B) Mixed chimeric mice were generated by injecting bone marrow cells from LysM-GFP⁺ WT mice and hck^−/− lyn^−/− fgr^−/− mice into lethally irradiated WT mice. Rolling velocities of 111 GFP⁺ (WT, filled circle) and 111 GFP⁻ (hck^−/− lyn^−/− fgr^−/−, open square) leukocytes in inflamed cremaster muscle venules of mixed chimeric mice (n = 4) treated with PTx and a monoclonal blocking antibody against P-selectin presented as cumulative histogram. (inset) Mean ± SEM. ^#, P < 0.05.