Bulk RNA-seq of human dermal LECs subjected to laminar or oscillatory shear stress. (A) Selected pathways from Enrichr analysis (GO terms) of DEGs upregulated in the LECs on the upstream sides of valve leaflets or upregulated in LECs on the downstream sides of valve leaflets (list of DEGs used for the enrichment analysis in Data S5). The vertical line represents the adjusted P value set at an FDR of 0.05. (B) Flow cytometry analysis of CD31 and PDPN expression of the three human dermal LEC donors used for bulk RNA-seq. Cells were gated on single cells, followed by viable cells, and the expression histograms shown in this panel. (C) Volcano plots of significant DEGs when comparing laminar or oscillatory shear stress to static control. (D) Heatmap of expression levels of selected genes in the bulk RNA-seq study. FDR, false discovery rate.