S1pr1 ^iΔLEC mice did not have obvious characteristics of inflammatory disease. (A) 10-mo-old S1pr1^f/f (TM@P1–7) and S1pr1^iΔLEC (TM@P1–7) littermates had comparable body weights irrespective of sex. (B) The number of Peyer’s patches in the guts (duodenum to cecum) of 10-mo-old S1pr1^f/f (TM@P1–7) and S1pr1^iΔLEC (TM@P1–7) littermates was counted and found to be comparable. (C and D) Spleen (C) and mesenteric LNs (D) of 10-mo-old S1pr1^f/f (TM@P1–7) and S1pr1^iΔLEC (TM@P1–7) littermates were comparable in size and shape. Statistics: (A) Each dot in the graph represents an individual animal. n = 4 male S1pr1^f/f, n = 4 male S1pr1^iΔLEC, n = 2 female S1pr1^f/f, and n = 4 female S1pr1^iΔLEC. Statistical significance was calculated for males using Mann–Whitney test; (B) n = 4 males and 1 female per genotype. Statistical significance was calculated using Mann–Whitney test; (C and D) images are representative of n = 3 animals/genotype/sex.