Resident cell types of the ALO dermal cortex. (A) UMAP plot of ALO scRNAseq data highlighting seven clusters that include resident cell types of the ALO cortex. (B) Schematic diagram of the ALO cortex (comparable to the area marked by the red box in the ALO confocal image at upper left), with the cell types represented by each of the highlighted clusters in panel A shown using the same colors. (C) Dot plot showing the relative expression of genes used to identify and characterize clusters corresponding to resident cell types of the ALO cortex. (D, F, H, J, L, N, P, and R) Confocal micrographs of the cortex of HCR stained (D, F, H, J, and N) or transgene-expressing (L, P [also shown in Fig. S3 G], and R) ALOs isolated from adult zebrafish. (E, G, I, K, M, O, Q, and S) Pseudocolored 2D sections from an array tomography section of the ALO cortex with the same cell types shown in the adjacent confocal image panels highlighted in pink. The confocal images and electron micrographs show surface epithelial cells (D and E), mid-level epithelial cells (F and G), basal epithelial cells (H and I), goblet cells (J and K), Merkel cells (L and M), chemosensory type 1 cells (N and O), chemosensory type 2 cells (P and Q), and club cells (R and S). HCR, n = 4; transgenic imaging, n = 4; TEM, n = 2. Scale bars = 10 µm (D, F, H, J, L, N, P, and R) or 5 µm (E, G, I, K, M, O, Q, and S).
