Figure 3.
Figure 3. Refer to the image caption for details.

scRNAseq of the ALO. (A) Confocal maximum intensity projection image of an ALO with nuclei labeled with Hoechst and a bar graph showing the average number of nuclei counted in four ALOs. (B) Schematic diagram showing the workflow for ALO scRNAseq; eight ALOs (from two males and two females) were processed. (C) Metrics for the ALO scRNAseq procedure. (D) UMAP plot of data from the ALO scRNAseq procedure, with 14 clusters annotated by cell identity. Scale bar = 300 µm (A).

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