Measurement of pulmonary lymphatic distension in LPS-induced acute lung inflammation. (A) Representative images of pulmonary lymphatic valves from steady-state controls and LPS-treated Rosa26^mTmG mice showing an approach for measuring lymphatic diameter. (B) Lymphatic vessel diameters at valve hinges and at sinuses immediately downstream of valves. (C) Sinus diameters divided by hinge diameters showing the relative distension of sinuses. Graphs show means ± SEM. P values are from (B) repeated-measures, two-way ANOVA with the Holm–Šídák test for the effect of LPS within vessel region groups; or (C) unpaired, two-tailed t test. Group sizes: n = 6.