Dynamics and diversity of leukocyte trafficking within intact pulmonary lymphatics. (A) Pulmonary lymphatic vessels from a steady-state control and LPS-treated Rosa26^mTmG mice at 24 h after onset of LPS-induced lung inflammation with arrowheads pointing to intralymphatic leukocytes. (B) Quantification of lymphatic flow of leukocytes. (C–H) Pulmonary lymphatics in Xcr1-Venus:Itgax-mCherry:Rosa26^mTmG mice at 24 h after LPS treatment with arrowheads pointing to Xcr1-Venus+ cDC1s, with cell types in lymphatics quantified in D and E using the mouse lines shown in this figure and in Fig. S2. Pulmonary lymphatic vessels from (F) Csf1r-ECFP:Rosa26^mTmG monocyte/macrophage reporter mouse or (G) MRP8-Cre:Rosa26^mTmG neutrophil reporter mouse 24 h after LPS treatment with (H) quantification of lymphatic flow of neutrophils. Graphs show means ± SEM. P values are from (B) the Kruskal–Wallis test with Dunn’s multiple comparisons to the 0-h (naïve) group; or (H) the Mann–Whitney test. Group sizes: (B) n = 4 (+1- and +6-h groups), n = 5 (0- and +24-h groups); (D and E) n = 3–5; (H) n = 5. Alv macs, alveolar macrophages.