Additional CITE-seq analysis. (A) UMAP plot of single cells clustered by transcriptomic data. Cells are colored according to clusters generated by the KNetL algorithm. (B) KNetL plot of single cells colored by cluster. (C) Heatmap of top 10 marker genes (rows) in individual cells (columns) for each cluster. (D) Heatmap showing select DEG between KrasG12D and WT cells within the HSC or MPP clusters. Average expression of DEG representing HSC-specific, lymphoid, proliferation-associated, or secreted products is shown according to the indicated color scale. (E and F) Feature plots showing the expression of lymphoid development–associated genes (E) and proliferation-associated genes (F) in individual cells on the KNetL plot. The KrasG12D-specific MPP cluster is highlighted. UMAP, Uniform Manifold Approximation and Projection.
