CD8 ⁺ T cell retention in the lymph node center is associated with CXCR3 desensitization. (A–C) P14 cell positioning in lymph nodes at d8 of acute LCMV infection in control and d0–1 IFNAR-blocked mice. Images and graphs are representative of three individual experiments with four to five mice in each group per experiment. (A) LSFM micrographs of intact lymph nodes. Images are 200-µm longitudinal slices through the lymph node center. Scale bars represent 200 µm. Images show GFP-P14 cells (yellow), B220 (B cells; cyan), and CD31 (vessels; magenta). (B) Graph summarizing density of P14 cells within the 3D lymph node, from periphery (EVF = 0) to center (EVF = 1). The black dashed line indicates multiple t tests between P14 cell density in the two treatment conditions for each EVF value. The red dashed line indicates P = 0.05. Data are the mean ± SEM. (C) Graphs summarizing average density ± SEM of P14 cells within indicated regions, IFRs (EVF 0.1–0.3), and T cell paracortex (EVF 0.7–0.9). (D–F) Analysis of P14 cells at d8 of acute LCMV infection in control and d0–1 IFNAR-blocked mice. Data are representative of two independent experiments with four to five mice per group in each experiment. Each dot in D and E represents a single mouse. Data are the mean ± SEM. Statistical differences were analyzed using one-way ANOVA. (D) Graph summarizing the gMFI of P14 cell CXCR3 expression. (E) Graph summarizing the upregulation of CXCR3 on P14 cell surface at different time points following cell isolation. (F) Migratory capacity of P14 cells at different concentrations of CXCL10. **P < 0.01, ***P < 0.001, ****P < 0.0001.