Early IFNAR blocking skews stem-like T cell differentiation without establishing chronic infection and exhaustion. (A–E) Analysis of P14 cells at d8 or d14 of acute LCMV infection, with or without IFNAR blocking at d0–1, or chronic LCMV Docile infection. Data are representative of three independent experiments with three to six mice per group in each experiment. Each dot in A–C and E represents a single mouse. Data are the mean ± SEM. Statistical differences were analyzed using one-way ANOVA tests. (A) Graphs summarizing the PFU from viable virus in spleens of mice in each infection condition. The dashed line indicates viral plaque LOD. (B) Graphs summarizing average frequencies of TIM-3 expression on P14 cells in each indicated group. (C) Graphs summarizing frequencies of stem-like cell populations within P14 cells in each group. (D and E) FlowSOM dimensionality reduction analysis of P14 cells generated in each infection condition. (D) FlowSOM dimensionality reduction analysis. tSNE plot displaying five FlowSOM-defined cell populations and denoted identities generated from differential surface antigen expression. (E) Frequency of each FlowSOM population within d8 SLAMF6⁺ P14 cells for each infection condition, and corresponding representative overlay of SLAMF6⁺ P14 cells displayed in tSNE plots. **P < 0.01, ***P < 0.001, ****P < 0.0001. Fig. S1 shows additional supporting data. LOD, limit of detection.