Figure S1.
IFNAR blocking at d0–1 of acute LCMV infection directs stem-like T cell differentiation without establishing chronic infection and exhaustion. Related to Figs. 1 and 2. (A–C) P14 cells generated in groups indicated in Fig. 1 A. Data are representative of two independent experiments with five mice per group in each experiment. Each dot in B represents a single mouse. Data are the mean ± SEM. Statistical differences were analyzed using one-way ANOVA tests. Data are representative of three independent experiments with four mice per group in each experiment. Average gMFI ± SEM for each graph are indicated. (A) Representative plots of P14 cells showing stem-like (TCF-1+SLAMF6+) T cell populations. (B) Graph summarizing frequencies in A. (C) Representative histograms of TEFF (KLRG1+; black histograms) and stem-like (TCF-1+SLAMF6+; gray histograms) P14 cell populations from treated control mice and stem-like (TCF-1+SLAMF6+; green histograms) P14 cells from d0–1 IFNAR-blocked mice for expression of CD44, CD127, CD62L, SCA-1, and PD-1. (D–H) Analysis of P14 cells from peripheral lymph nodes of mice at d8 or d14 of acute LCMV Armstrong with or without IFNAR block at d0–1, or chronic LCMV Docile infection. Data are representative of three independent experiments with four mice per group in each experiment. Each dot in H represents a single mouse. Data are the mean ± SEM. Statistical differences were analyzed using one-way ANOVA tests. (D) Representative plots of TIM-3 expression on P14 cells within each infection condition. (E) Representative flow cytometry plots of TEFF (KLRG1+SLAMF6−) and stem-like (KLRG1−SLAMF6+) T cell populations within P14 cells from each group. (F) Overlay of marker expression heat maps on t-distributed stochastic neighbor embedding (tSNE) plot generated by FlowSOM. (G) FlowSOM heat map determining distinction of discreet populations. (H) Frequency of each FlowSOM population within d14 SLAMF6+ P14 cells for each infection condition, and corresponding representative overlay of SLAMF6+ P14 cells displayed in tSNE plots. *P < 0.05, **P < 0.01, ****P < 0.0001.

IFNAR blocking at d0–1 of acute LCMV infection directs stem-like T cell differentiation without establishing chronic infection and exhaustion. Related to Figs. 1 and 2. (A–C) P14 cells generated in groups indicated in Fig. 1 A. Data are representative of two independent experiments with five mice per group in each experiment. Each dot in B represents a single mouse. Data are the mean ± SEM. Statistical differences were analyzed using one-way ANOVA tests. Data are representative of three independent experiments with four mice per group in each experiment. Average gMFI ± SEM for each graph are indicated. (A) Representative plots of P14 cells showing stem-like (TCF-1+SLAMF6+) T cell populations. (B) Graph summarizing frequencies in A. (C) Representative histograms of TEFF (KLRG1+; black histograms) and stem-like (TCF-1+SLAMF6+; gray histograms) P14 cell populations from treated control mice and stem-like (TCF-1+SLAMF6+; green histograms) P14 cells from d0–1 IFNAR-blocked mice for expression of CD44, CD127, CD62L, SCA-1, and PD-1. (D–H) Analysis of P14 cells from peripheral lymph nodes of mice at d8 or d14 of acute LCMV Armstrong with or without IFNAR block at d0–1, or chronic LCMV Docile infection. Data are representative of three independent experiments with four mice per group in each experiment. Each dot in H represents a single mouse. Data are the mean ± SEM. Statistical differences were analyzed using one-way ANOVA tests. (D) Representative plots of TIM-3 expression on P14 cells within each infection condition. (E) Representative flow cytometry plots of TEFF (KLRG1+SLAMF6−) and stem-like (KLRG1−SLAMF6+) T cell populations within P14 cells from each group. (F) Overlay of marker expression heat maps on t-distributed stochastic neighbor embedding (tSNE) plot generated by FlowSOM. (G) FlowSOM heat map determining distinction of discreet populations. (H) Frequency of each FlowSOM population within d14 SLAMF6+ P14 cells for each infection condition, and corresponding representative overlay of SLAMF6+ P14 cells displayed in tSNE plots. *P < 0.05, **P < 0.01, ****P < 0.0001.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal