Figure S4.
Hemicentin-1 VWA domain, K14-encoding cDNA constructs expression in HeLa cells and HMCN1 silencing efficiency. (A) Hemicentin-1 VWA domain and K14 (KRT14)-expressing cDNA constructs were transfected in HeLa cells. Gene expression was quantified using RT-qPCR. Results were normalized to GAPDH mRNA, represent the mean ± SE of two experiments and are expressed as fold change of mRNA expression in cells transfected with an empty vector (one-way ANOVA; **P < 0.01, ***P < 0.005). (B)HMCN1 mRNA levels were quantified by RT-qPCR in primary human keratinocytes (KC) or fibroblasts (FB) transfected with HMCN1-specific (siHMCN1) or scramble (siControl) siRNAs. Results were normalized to GAPDH mRNA, represent the mean ± SE of three experiments, and are expressed as a percentage of HMCN1 mRNA expression in cells transfected with siControl (two-way t test; ***P < 0.005).

Hemicentin-1 VWA domain, K14-encoding cDNA constructs expression in HeLa cells and HMCN1 silencing efficiency. (A) Hemicentin-1 VWA domain and K14 (KRT14)-expressing cDNA constructs were transfected in HeLa cells. Gene expression was quantified using RT-qPCR. Results were normalized to GAPDH mRNA, represent the mean ± SE of two experiments and are expressed as fold change of mRNA expression in cells transfected with an empty vector (one-way ANOVA; **P < 0.01, ***P < 0.005). (B)HMCN1 mRNA levels were quantified by RT-qPCR in primary human keratinocytes (KC) or fibroblasts (FB) transfected with HMCN1-specific (siHMCN1) or scramble (siControl) siRNAs. Results were normalized to GAPDH mRNA, represent the mean ± SE of three experiments, and are expressed as a percentage of HMCN1 mRNA expression in cells transfected with siControl (two-way t test; ***P < 0.005).

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