Discrimination of CD45 ⁺ , KIT ⁺ , or CD45 ⁺ KIT ⁺ hematopoietic cells in cells in FL. (A) Representative single-stack IF of an E12.5 FL section with DNA (white), Ter119, CD71 (green), CD45 (red), and KIT (blue). Scale bar, 200 μm. (a′ and a″) Inserts a′ and a″ represent enlarged 3D views (10 μm projections) of the selected regions. Scale bars: 100 μm (a′), 20 μm (a″). Blue (upper), red (middle), and purple (lower) arrowheads indicate an example of a KIT⁺, CD45⁺, or KIT⁺CD45⁺ (DP) cell, respectively. Images with cubes are 3D projections.