Discrimination of the FL’s stromal compartments in situ. (A) Representative 3D view (30 μm projection) IF of an E13.5 FL section with LHX2 (green), CD146 (red), and PDGFRα (blue). (B) Representative 3D view (50 μm projection) IF of an E16.5 FL section with Desmin (green) and CD146 (red). (C) Representative 3D view (57 μm projection) IF of an E18.5 FL section with Desmin (green), CD146 (red), and PDGFRα (blue). (D) Representative 3D view (10 μm) IF of an E18.5 FL section with DNA (white), NG2 (green), αSMA (red), and EpCAM (blue). (d′) The insert d′ shows the selected region with the channels separated. (E) Representative 3D view (20 μm projection) IF of an E12.5 FL section with DNA (white) and NG2 (green). (F) Representative 3D view (50 μm projection) IF of an E18.5 FL section with ALCAM (green) and CD146 (red). (G) Representative 3D view (50 μm projection) IF of an E12.5 FL section with DNA (white), Gp38 (red), and CD146 (green). (H) Representative 3D view (30 μm projection) IF of an E14.5 FL section with CD146 (red), WT1 (green), and PDGFRα (blue). (I) Single stack IF of an E13 FL section with DNA (white), LHX2 (green), CD146 (red), and PDGFRα (blue). (J) Single stack IF of an E12.5 FL section with DNA (white), CD146 (red), and Gp38 (blue). (K) 3D view (50 μm projection) IF of an E12.5 FL section with CD146 (red), LHX2 (green), and PDGFRα (blue). (L) Representative single stack IF of an E18.5 FL section with DNA (white) and E-Cadh (red). Arrowheads point to the mesothelium. Scale bars, 100 μm. CV, central vein; PV, portal vein. Images with cubes are 3D projections.