Creation process of a molecular simulation system consisting of the PTx2–hNa _V 1.7 activated VSD II complex embedded in a POPC bilayer and solvated with aqueous 0.15 M NaCl solution. PTx2 and VSD II are shown as green and dark gray surfaces, respectively. The lipid membrane composed of POPC lipids is shown in blue sticks. Water is displayed as a transparent surface. Na⁺ and Cl^– ions are displayed as purple and gold spheres, respectively.