Structural comparison of different conformational states of hNa V 1.7 VSD II and IV. (A) Snapshots of deactivated (left) and activated (right) VSD II structures. For each state, S1–S4 VSD segments are shown, highlighting the gating charge residues (R1, R2, R3, K4) and the hydrophobic constriction site (HCS) in yellow. The distance between the Cα atoms of each gating charge in each state after superimposition of the VSDs is shown. (B) Global view of cryo-EM NaV1.7 structure from PDB accession no. 6J8J (Shen et al., 2019) highlights the VSD II and IV positions. (C) Snapshots of deactivated (left) and activated (right) VSD IV structures (same information as in panel A with the gating charge residues R1, R2, R3, R4, and R5 highlighted).
