Water occupancy along the channel pore. Plot showing convergence of water density distribution during different trajectory slabs (of 50 ns) for WT-C (PDB ID: 7QKO; left) and triple mutated system (7QKO-bdeL9′S; right) along the pore, calculated within a cylinder of radius 5 Å along the pore axis. Note the water density profile in the hydrophobic 9′–16′ region remains conserved with time.