Figure S3. Representative interaction... | Rockefeller University Press

Figure S3.

Representative interaction energy measurement at 12′ and 13′ positions. (a and b) MCA to estimate Δ2G0 between 12′T in the α and β subunits (a) and between 13′V in the β and ε subunits (b). Representative single-channel currents and dwell-time distribution histograms are shown for the WT, mutant A, B, and double mutant AB. The corresponding free energy changes (see Tables S4 and S5) and scale bars are shown.

Representative interaction energy measurement at 12′ and 13′ positions. (a and b) MCA to estimate Δ²G₀ between 12′T in the α and β subunits (a) and between 13′V in the β and ε subunits (b). Representative single-channel currents and dwell-time distribution histograms are shown for the WT, mutant A, B, and double mutant AB. The corresponding free energy changes (see Tables S4 and S5) and scale bars are shown.