Figure S2 The extent of fast... | Rockefeller University Press

Figure S2

$The extent of fast inactivation depends on the duration and amplitude of applied depolarizing pulse.(A) Sample current traces of mutants in response to depolarization pulse from holding potential −100 mV to +60 mV with 10 mV increments (left). Enlarged tail currents visualize hooks on the tail (right). (B) Analysis of fractional inactivation depending on activation potential. Tail currents were fitted to two- or three-exponential functions and extrapolated to the beginning of the prepulse segment to determine overall tail amplitude; n = 4–6, error bars represent ±SEM. (C and D) Dependency of the fast inactivation of wild type and mutant KCNQ1 channels on the duration of activating pulse. (C) Exemplary current traces of KCNQ1 wild type and mutant channels elicited by protocol with various duration of activation at +60 mV. Interpulse interval was adjusted to obtain maximal overlay of activation components. (D) The dependency of the inactivation fraction from the duration of activating pulse; n = 3–6, error bars represent ±SEM.$

The extent of fast inactivation depends on the duration and amplitude of applied depolarizing pulse. (A) Sample current traces of mutants in response to depolarization pulse from holding potential −100 mV to +60 mV with 10 mV increments (left). Enlarged tail currents visualize hooks on the tail (right). (B) Analysis of fractional inactivation depending on activation potential. Tail currents were fitted to two- or three-exponential functions and extrapolated to the beginning of the prepulse segment to determine overall tail amplitude; n = 4–6, error bars represent ±SEM. (C and D) Dependency of the fast inactivation of wild type and mutant KCNQ1 channels on the duration of activating pulse. (C) Exemplary current traces of KCNQ1 wild type and mutant channels elicited by protocol with various duration of activation at +60 mV. Interpulse interval was adjusted to obtain maximal overlay of activation components. (D) The dependency of the inactivation fraction from the duration of activating pulse; n = 3–6, error bars represent ±SEM.